Hydroxamate-induced spectral perturbations of cobalt Aeromonas aminopeptidase.

Photochemical inactivation of Aeromonas aminopeptidase by 2,3-butanedione.

Isolation of the Leucine Aminopeptidase Gene from Aeromonas proteolytica

The leucine aminopeptidase of Aeromonas proteolytica (EC 3.4.11.10) is a monomeric metalloenzyme having the capacity to bind two Zn2+ atoms in the active site. Structural information of this relatively small aminopeptidase that could illuminate the catalytic mechanism of the metal ions is lacking; hence, we have obtained sequences from the purified enzyme, cloned the corresponding gene, and ex...

Spectral Analysis of Microwave Background Perturbations Induced by Cosmic Strings

Using a simple analytic model based on seed superposition we obtain the spectrum of microwave background perturbations induced by cosmic strings on all angular scales larger than about 2 armin. We assume standard recombination in an Einstein de Sitter universe with h = 1/2 and study the fluctuation spectrum along a great circle in the sky. Doppler and potential perturbations on the last scatter...

Aeromonas aminopeptidase. Improved isolation and some physical properties.

The extracellular aminopeptidase of Aeromonas proteofytica was purified to homogeneity by a simplified procedure that proved feasible for the isolation of gram quantities of this enzyme. Precipitation with ammonium sulfate and acetone, heating at 70”, and chromatography on Sephadex G-75 and DEAE-Sephadex yielded a product that showed a high degree of purity by polyacrylamide and free boundary e...

Leucine aminopeptidase (bovine lens). The relative binding of cobalt and zinc to leucine aminopeptidase and the effect of cobalt substitution on specific activity.

Prolonged incubation of zinc-zinc leucine aminopeptidase (bovine lens) (EC 3.4.1.1) with 0.05 M CoCl2 and M KCl in 0.2 M N-ethylmorpholine-HCl at pH 7.5 and 37 degrees yields an active enzyme in which 2 g atoms of Co2+ per 54,000 dalton subunit have replaced the Zn2+. Incubation of cobalt-cobalt leucine aminopeptidase with various AnCl2 concentrations or zinc-zinc leucine aminopeptidase with va...